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Article

Identification of the sequences required for chromosomal replicator function in Kluyveromyces lactis.

Molecular microbiology - 1 Mar 2004

Irene Carmela, Maciariello Clelia, Cioci Francesco, Camilloni Giorgio, Newlon Carol S, Fabiani Lucia

Abstract excerpt

The analysis of replication intermediates of a Kluyveromyces lactis chromosomal autonomous replicating sequence (ARS), KARS101, has shown that it is active as a chromosomal replicator. KARS101 contains a 50 bp sequence conserved in two other K. lactis ARS elements. The deletion of the conserved sequence in KARS101 completely abolished replicator activity, in both the plasmids and the chromosome. Gel shift assays...

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PMID: 14982634
DOI: 10.1046/j.1365-2958.2003.03914.x

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