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Article

Overproduction in Escherichia coli and characterization of yeast replication factor C lacking the ligase homology domain.

The Journal of biological chemistry - 12 May 2000

Gomes X V, Gary S L, Burgers P M

Abstract excerpt

Eukaryotic replication factor C (RF-C) is a heteropentameric complex that is required to load the replication clamp proliferating cell nuclear antigen onto primed DNA. Saccharomyces cerevisiae RF-C is encoded by the genes RFC1-RFC5. The RFC1 gene was cloned under control of the strong inducible bacteriophage T7 promoter, yet induction did not yield detectable Rfc1p. However, a truncated form of RFC1 deleted for...

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Source

Identifiers

PMID: 10799539
DOI: 10.1074/jbc.275.19.14541

Genes

RFC1
RFC5

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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