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Article

Nonglucosylated oligosaccharides are transferred to protein in MI8-5 Chinese hamster ovary cells.

Glycobiology - 1 Jan 1999

Quellhorst G J, O'Rear J L, Cacan R, Verbert A, Krag S S

Abstract excerpt

A CHO mutant MI8-5 was found to synthesize Man9-GlcNAc2-P-P-dolichol rather than Glc3Man9GlcNAc2-P-P-dolichol as the oligosaccharide-lipid intermediate in N-glycosylation of proteins. MI8-5 cells were incubated with labeled mevalonate, and the prenol was found to be dolichol. The mannose-labeled oligosaccharide released from oligosaccharide-lipid of MI8-5 cells was analyzed by HPLC and alpha-mannosidase...

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PMID: 9884408
DOI: 10.1093/glycob/9.1.65

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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