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Article

Chinese hamster ovary cells with reduced hexokinase activity maintain normal GDP-mannose levels.

Journal of cellular biochemistry - 1 Jan 1999

O'Rear J L, Scocca J R, Walker B K, Kaiden A, Krag S S

Abstract excerpt

Parental Chinese hamster ovary (CHO) cells were mutagenized and subjected first to a mannose suicide selection technique and second to a screen of individual colonies grown on polyester discs for reduced mannose incorporation into protein. The incorporation of radioactivity for the selection and the screen was conducted at 41.5 degrees C instead of the normal growth temperature of 34 degrees C in order to allow...

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PMID: 10025667
DOI: 10.1002/(sici)1097-4644(19990101)72:1<56::aid-jcb7>3.0.co;2-h

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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