Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Three enzymes involved in oligosaccharide-lipid assembly in Chinese hamster ovary cells differ in lipid substrate preference.

Journal of lipid research - 1 Oct 1994

McLachlan K R, Krag S S

Abstract excerpt

Initial steps in N-linked glycosylation involve formation of a large oligosaccharide structure on a lipid carrier, dolichyl phosphate. We have previously characterized Chinese hamster ovary (CHO) glycosylation mutants (Lec9 cells) that utilize the polyisoprenoid lipid polyprenyl phosphate rather than dolichyl phosphate in these glycosylation reactions. Polyprenyl phosphate differs from dolichyl phosphate only in...

Topics

Animals
CHO Cells
Carbohydrate Sequence
Cells, Cultured
Cricetinae
Glucosyltransferases
Glycosylation
Lipids
Mannosyltransferases
Molecular Sequence Data
N-Acetylglucosaminyltransferases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oligosaccharides

Phenotype

Substrate Specificity

Source

Identifiers

PMID: 7852863

Genes

CPD

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7852863-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Three enzymes involved in oligosaccharide-lipid assembly in Chinese hamster ovary cells differ in lipid substrate preference.PMID 7852863Read ↗

Lec9 CHO glycosylation mutants are defective in the synthesis of dolichol.Semantic proximity Read ↗

Reduced utilization of Man5GlcNAc2-P-P-lipid in a Lec9 mutant of Chinese hamster ovary cells: analysis of the steps in oligosaccharide-lipid assembly.Semantic proximity Read ↗

Mannolipid donor specificity of glycosylphosphatidylinositol mannosyltransferase-I (GPIMT-I) determined with an assay system utilizing mutant CHO-K1 cells.Semantic proximity Read ↗

Regulation of glycosylation. Three enzymes compete for a common pool of dolichyl phosphate in vivo.Semantic proximity Read ↗

Low expression of lipid-linked oligosaccharide due to a functionally altered Dol-P-Man synthase reduces protein glycosylation in cAMP-dependent protein kinase deficient Chinese hamster ovary cells.Semantic proximity Read ↗

The N-glycosylation defect in Lec5 and Lec9 CHO cells is caused by absence of the DHRSX geneSemantic proximity Read ↗

Stepwise assembly of the lipid-linked oligosaccharide in the endoplasmic reticulum of Saccharomyces cerevisiae: identification of the ALG9 gene encoding a putative mannosyl transferase.Semantic proximity Read ↗

Nonglucosylated oligosaccharides are transferred to protein in MI8-5 Chinese hamster ovary cells.Semantic proximity Read ↗

Expression of functional bacterial undecaprenyl pyrophosphate synthase in the yeast rer2{Delta} mutant and CHO cells.Semantic proximity Read ↗

Reciprocal relationship between alpha1,2 mannosidase processing and reglucosylation in the rough endoplasmic reticulum of Man-P-Dol deficient cells.Semantic proximity Read ↗

Expression cloning of a novel suppressor of the Lec15 and Lec35 glycosylation mutations of Chinese hamster ovary cells.Semantic proximity Read ↗

Lecithin-cholesterol acyltransferase: effects of mutagenesis at N-linked oligosaccharide attachment sites on acyl acceptor specificity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.