Article
Effect of plating medium and phage storage on mutant frequency and titer in the lambda cII transgenic mutation assay.
Environmental and molecular mutagenesis - 1 Jan 1998
Zimmer D M, Harbach P R, Mattes W B, Aaron C S
Abstract excerpt
We examined several experimental parameters of the lambda cI/cII transgenic mutation assay. In the assay, clear plaque lambda phage mutants are identified in a positive selection scheme following rescue of the lambda/LIZ shuttle vector from frozen tissues of Big Blue" transgenic mice. Mutant freq...
Topics
- Animals
- Bacteriophage lambda
- Genotype
- Liver
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Mutation
- Spleen