Article
Mutant frequencies and mutation spectra of dimethylnitrosamine (DMN) at the lacI and cII loci in the livers of Big Blue transgenic mice.
Mutation research - 18 Sept 2000
Shane B S, Smith-Dunn D L, de Boer J G, Glickman B W, Cunningham M L
Abstract excerpt
The lacI gene in Big Blue transgenic rodents has traditionally been used as a surrogate gene for in vivo mutations. Recently, a more efficient and less expensive assay involving direct selection in the smaller lambda cII gene has been developed. Little is known, however, about the comparative sensitivity of the two loci or their influence on the recovered mutation spectrum following mutagen treatment. We have...
Topics
- Animals
- Bacterial Proteins
- Base Sequence
- DNA Primers
- Dimethylnitrosamine
- Escherichia coli Proteins
- Lac Repressors
- Liver
- Male
- Mice
- Mice, Transgenic
- Mutagens
- Mutation
- Repressor Proteins
- Transcription Factors
- Viral Proteins