Asp34 of PvuII endonuclease is directly involved in DNA minor groove recognition and indirectly involved in catalysis.

doi:10.1006/jmbi.1998.2269

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Article

Asp34 of PvuII endonuclease is directly involved in DNA minor groove recognition and indirectly involved in catalysis.

Journal of molecular biology - 18 Dec 1998

Horton J R, Nastri H G, Riggs P D, Cheng X

Abstract excerpt

The PvuII restriction endonuclease is a homodimer that recognizes and cleaves the DNA sequence 5'-CAGCTG-3' in double-stranded DNA, and the structure of this enzyme has been reported. In the wild-type enzyme, Asp34 interacts with the internal guanine of the recognition sequence on the minor groov...

Topics

Aspartic Acid
Base Pairing
Binding Sites
Catalytic Domain
DNA
Deoxyribonucleases, Type II Site-Specific
Iodine
Models, Molecular
Mutation
Nucleic Acid Conformation
Protein Conformation
Structure-Activity Relationship

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