Article
G350 of Escherichia coli RNase P RNA contributes to Mg2+ binding near the active site of the enzyme.
Gene - 10 Jul 2002
Rasmussen Terri A, Nolan James M
Abstract excerpt
G350 of Escherichia coli RNase P RNA is a highly conserved residue among all bacteria and lies near the known magnesium binding site for the RNase P ribozyme, helix P4. Mutations at G350 have a dramatic effect on substrate cleavage activity for both RNA alone and holoenzyme; the G350C mutation has the most severe phenotype. The G350C mutation also inhibits growth of cells that express the mutant RNA in vivo under...
Topics
- Base Sequence
- Binding Sites
- Binding, Competitive
- Cell Division
- Edetic Acid
- Endoribonucleases
- Escherichia coli
- Escherichia coli Proteins
- Genetic Complementation Test
- Guanosine
- Holoenzymes
- Magnesium
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA, Bacterial
- RNA, Catalytic
- Ribonuclease P