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Article

Covalent joining of the subunits of a homodimeric type II restriction endonuclease: single-chain PvuII endonuclease.

Journal of molecular biology - 25 May 2001

Simoncsits A, Tjörnhammar M L, Raskó T, Kiss A, Pongor S

Abstract excerpt

The PvuII restriction endonuclease has been converted from its natural homodimeric form into a single polypeptide chain by tandemly linking the two subunits through a short peptide linker. The arrangement of the single-chain PvuII (sc PvuII) is (2-157)-GlySerGlyGly-(2-157), where (2-157) represents the amino acid residues of the enzyme subunit and GlySerGlyGly is the peptide linker. By introducing the...

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Identifiers

PMID: 11491304
DOI: 10.1006/jmbi.2001.4651

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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