Article
Probing the ligand binding domain of the GluR2 receptor by proteolysis and deletion mutagenesis defines domain boundaries and yields a crystallizable construct.
Protein science : a publication of the Protein Society - 1 Dec 1998
Chen G Q, Sun Y, Jin R, Gouaux E
Abstract excerpt
Ionotropic glutamate receptors constitute an important family of ligand-gated ion channels for which there is little biochemical or structural data. Here we probe the domain structure and boundaries of the ligand binding domain of the AMPA-sensitive GluR2 receptor by limited proteolysis and delet...
Topics
- Amino Acid Sequence
- Binding Sites
- Chymotrypsin
- Crystallization
- Crystallography, X-Ray
- Glutamic Acid
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Folding
- Receptors, AMPA
- Recombinant Proteins
- Sequence Deletion
- Trypsin