Article
Glutamate binding and conformational flexibility of ligand-binding domains are critical early determinants of efficient kainate receptor biogenesis.
The Journal of biological chemistry - 22 May 2009
Gill Martin B, Vivithanaporn Pornpun, Swanson Geoffrey T
Abstract excerpt
Intracellular glutamate binding within the endoplasmic reticulum (ER) is thought to be necessary for plasma membrane expression of ionotropic glutamate receptors. Here we determined the importance of glutamate binding to folding and assembly of soluble ligand-binding domains (LBDs), as well as full-length receptors, by comparing the secretion of a soluble GluR6-S1S2 protein versus the plasma membrane localization...
Topics
- Animals
- COS Cells
- Calnexin
- Cell Membrane
- Chlorocebus aethiops
- Disulfides
- Dithiothreitol
- Endoplasmic Reticulum
- Glutamic Acid
- Glycosylation
- Humans
- Ligands
- Mutant Proteins
- Mutation
- Phenotype