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Article

Catalytic and binding mutants of the junction-resolving enzyme endonuclease I of bacteriophage t7: role of acidic residues.

Nucleic acids research - 15 Jan 1999

Parkinson M J, Pöhler J R, Lilley D M

Abstract excerpt

Endonuclease I is a 149 amino acid protein of bacteriophage T7 that is a Holliday junction-resolving enzyme, i.e. a four-way junction-selective nuclease. We have performed a systematic mutagenesis study of this protein, whereby all acidic amino acids have been individually replaced by other residues, mainly alanine. Out of 21 acidic residues, five (Glu20, Glu35, Glu65, Asp55 and Asp74) are essential. Replacement...

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Identifiers

PMID: 9862997
DOI: 10.1093/nar/27.2.682
PMCID: PMC148232

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