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Article

Identification of amino acids of endonuclease VII essential for binding and cleavage of cruciform DNA.

European journal of biochemistry - 1 May 1997

Golz S, Christoph A, Birkenkamp-Demtröder K, Kemper B

Abstract excerpt

Endonuclease VII is a Holliday-structure-resolving enzyme of bacteriophage T4. The active protein is a homodimer with 157 amino acids/monomer. An amber mutation (amE727 in codon 151) inactivates the nuclease completely, indicating the importance of the seven C-terminal amino acids for nucleolytic activity. The influence of these amino acids on cruciform-DNA binding and cleavage was investigated through functional...

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PMID: 9182992
DOI: 10.1111/j.1432-1033.1997.00573.x

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