Article
Two basic residues, Lys-107 and Lys-118, of RuvC resolvase are involved in critical contacts with the Holliday junction for its resolution.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Oct 2000
Yoshikawa M, Iwasaki H, Kinoshita K, Shinagawa H
Abstract excerpt
BACKGROUND: Crystallographic and mutational studies of Escherichia coli RuvC Holliday junction resolvase have revealed that a catalytic site of each subunit is composed of four acidic residues at the bottom of the putative DNA-binding cleft, whose surface contains eight basic residues. RESULTS: To elucidate the functional roles of the basic residues on the cleft surface, we constructed a series of mutant ruvC...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.