Substitution of basic amino acids within endonuclease V enhances nontarget DNA binding.

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Article

Substitution of basic amino acids within endonuclease V enhances nontarget DNA binding.

The Journal of biological chemistry - 25 Mar 1991

Nickell C, Anderson W F, Lloyd R S

Abstract excerpt

Several DNA-interactive proteins, including the DNA repair enzyme T4 endonuclease V, have been shown to locate their target recognition sites utilizing an electrostatically mediated facilitated diffusion mechanism. Previous work indicates that a decrease in the affinity of endonuclease V for nontarget DNA results in an increased nontarget dissociation rate. This study was designed to investigate the effect of an...

Topics

Amino Acids
Animals
Bacteriophages
Base Sequence
Cattle
DNA
DNA, Bacterial
DNA, Viral
Deoxyribonuclease (Pyrimidine Dimer)
Endodeoxyribonucleases
Escherichia coli
Genes, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genes, Viral

Kinetics

Molecular Sequence Data

Mutagenesis, Site-Directed

Mutation

Plasmids

Source

Open in PubMed

Identifiers

PMID: 2005104

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2005104-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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