Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild type gene.

doi:10.1007/BF00280302

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Article

Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild type gene.

Molecular & general genetics : MGG - 1 Dec 1991

Anaya N, Roncero M I

Abstract excerpt

A transformation system has been developed for Mucor circinelloides, by direct cloning of a wild-type methionine gene that complements the auxotrophic mutation. The marker gene isolated was associated with an autonomous replication sequence (ARS) functional in this zygomycete. Southern hybridisat...

Topics

Blotting, Southern
Cloning, Molecular
DNA Replication
DNA, Fungal
Genes, Fungal
Genomic Library
Methionine
Mitosis
Mucor
Mutation
Plasmids
Transformation, Genetic

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