Article
Cloning and sequence analysis of the Mucor circinelloides pyrG gene encoding orotidine-5'-monophosphate decarboxylase: use of pyrG for homologous transformation.
Gene - 1 Jul 1992
Benito E P, Díaz-Mínguez J M, Iturriaga E A, Campuzano V, Eslava A P
Abstract excerpt
A 3.2-kb BamHI genomic DNA fragment containing the pyrG gene of Mucor circinelloides was isolated by heterologous hybridization using a pyrG cDNA clone of Phycomyces blakesleeanus as the probe. The complete nucleotide sequence of the M. circinelloides pyrG gene encoding orotidine-5'-monophosphate decarboxylase (OMPD) was determined and the transcription start points (tsp) were mapped by primer extension analysis....
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Cloning, Molecular
- Genetic Complementation Test
- Introns
- Molecular Sequence Data
- Mucor
- Mutation
- Orotidine-5'-Phosphate Decarboxylase