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Article

A multicopy vector system for genetic studies in Mucor circinelloides and other zygomycetes.

Molecular genetics and genomics : MGG - 1 Jun 2004

Appel K F, Wolff A M, Arnau J

Abstract excerpt

Transformation of Mucor circinelloides is routinely achieved by using a plasmid containing the wild-type leuA gene to complement the leucine requirement of an auxotrophic host strain. As is the case for other zygomycetes, the transforming DNA is usually not integrated into the genome of M. circinelloides, but is maintained as an autonomously replicating plasmid. However, even under selective conditions, the...

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PMID: 15088140
DOI: 10.1007/s00438-004-1008-6

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