Heterospecific cloning of Arabidopsis thaliana cDNAs by direct complementation of pyrimidine auxotrophic mutants of Saccharomyces cerevisiae. I. Cloning and sequence analysis of two cDNAs catalysing the second, fifth and sixth steps of the de novo pyrimidine biosynthesis pathway.

doi:10.1007/BF00280183

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Article

Heterospecific cloning of Arabidopsis thaliana cDNAs by direct complementation of pyrimidine auxotrophic mutants of Saccharomyces cerevisiae. I. Cloning and sequence analysis of two cDNAs catalysing the second, fifth and sixth steps of the de novo pyrimidine biosynthesis pathway.

Molecular & general genetics : MGG - 8 Jul 1994

Nasr F, Bertauche N, Dufour M E, Minet M, Lacroute F

Abstract excerpt

An Arabidopsis thaliana cDNA library was used to complement Saccharomyces cerevisiae pyrimidine auxotrophic mutants. Mutants in all but one (carbamylphosphate synthetase) of the six steps in the de novo pyrimidine biosynthetic pathway could be complemented. We report here the cloning, sequencing...

Topics

Amino Acid Sequence
Animals
Arabidopsis
Aspartate Carbamoyltransferase
Base Sequence
Catalysis
Cloning, Molecular
DNA, Complementary
Genetic Complementation Test
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Ornithine Carbamoyltransferase

Orotate Phosphoribosyltransferase

Orotidine-5'-Phosphate Decarboxylase

Pyrimidines

Saccharomyces cerevisiae

Sequence Homology, Amino Acid

Source

Open in PubMed

Identifiers

PMID: 8041358
DOI: 10.1007/BF00280183

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8041358-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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