Article
Translational coupling in the pyrF operon of Salmonella typhimurium.
Molecular & general genetics : MGG - 1 Jul 1990
Theisen M, Neuhard J
Abstract excerpt
The pyrF gene, encoding the sixth enzyme of pyrimidine biosynthesis in Salmonella typhirmurium, appears to be the first gene of an operon. The second gene, orfF, encodes a 11.5 kDa polypeptide of unknown function. To study the regulation of orfF expression directly, transcriptional and translational fusions of orfF to galK and lacZ, respectively, were constructed and the level of expression of the reporter genes...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Codon
- DNA, Bacterial
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data
- Mutation
- Operon
- Orotidine-5'-Phosphate Decarboxylase
- Plasmids