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Article

Isolation, characterization and transformation, by autonomous replication, of Mucor circinelloides OMPdecase-deficient mutants.

Molecular & general genetics : MGG - 28 Jul 1995

Benito E P, Campuzano V, Lŏpez-Matas M A, De Vicente J I, Eslava A P

Abstract excerpt

Pyrimidine auxotrophs of Mucor circinelloides were isolated after mutagenesis with nitrosoguanidine and selected for resistance to 5-fluoroorotate. These mutants were genetically and biochemically characterized and found to be deficient either in orotidine-5'-monophosphate decarboxylase (OMPdecase) activity or in orotate phosphoribosyl transferase (OPRTase) activity. Different circular DNA molecules containing...

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PMID: 7651335
DOI: 10.1007/BF02190793

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