Article
Gyrase and Topo IV modulate chromosome domain size in vivo.
Molecular microbiology - 1 Sept 1998
Staczek P, Higgins N P
Abstract excerpt
In bacteria, DNA supercoil movement is restricted to subchromosomal regions or 'domains.' To elucidate the nature of domain boundaries, we analysed reaction kinetics for gammadelta site-specific resolution in six chromosomal intervals ranging in size from 14 to 90 kb. In stationary cultures of Salmonella typhimurium, resolution kinetics were rapid for both short and long intervals, suggesting that random...
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