Article
Surveying a supercoil domain by using the gamma delta resolution system in Salmonella typhimurium.
Journal of bacteriology - 1 May 1996
Higgins N P, Yang X, Fu Q, Roth J R
Abstract excerpt
A genetic system was developed to investigate the supercoil structure of bacterial chromosomes. New res-carrying transposons were derived from MudI1734 (MudJr1 and MudJr2) and Tn10 (Tn10dGn). The MudJr1 and MudJr2 elements each have a res site in opposite orientation so that when paired with a Tn10dGn element in the same chromosome, one MudJr res site will be ordered as a direct repeat. Deletion formation was...
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