Article
Efficient production of intact human parathyroid hormone in a Saccharomyces cerevisiae mutant deficient in yeast aspartic protease 3 (YAP3).
Applied microbiology and biotechnology - 1 Aug 1998
Kang H A, Kim S J, Choi E S, Rhee S K, Chung B H
Abstract excerpt
When human parathyroid hormone (hPTH) is expressed as a secretory product in yeast, the main problem is the aberrant proteolytic cleavage that reduces the yield of intact protein. To overcome this problem, we developed an hPTH expression system using a host strain in which the YAP3 gene encoding yeast aspartic protease 3 (YAP3) was disrupted. After 48 h of culture, most of the hPTH secreted by the yap3 disruptant...
Topics
- Aspartic Acid Endopeptidases
- Humans
- Mutation
- Parathyroid Hormone
- Pepstatins
- Peptide Fragments
- Protease Inhibitors
- Protein Processing, Post-Translational
- Recombinant Proteins
- Saccharomyces cerevisiae