Article
In vivo processing of nonanchored Yapsin 1 (Yap3p).
Archives of biochemistry and biophysics - 15 Mar 2000
Olsen V, Loh Y P
Abstract excerpt
A C-terminally truncated form of yapsin 1 (yeast aspartic protease 3) was overexpressed in yeast and its processing through the secretory pathway was followed by pulse-labeling and immunoprecipitation studies. In the soluble cell extract, three forms of yapsin 1-87, 74, and 18 kDa-were found. Identification of these forms of yapsin 1 using different antisera suggests that the 87-kDa form is pro-yapsin 1, which is...
Topics
- Adenosine Triphosphatases
- Amino Acid Sequence
- Aspartic Acid Endopeptidases
- Binding Sites
- Catalysis
- Culture Media, Conditioned
- Endoplasmic Reticulum
- Fungal Proteins
- Genes, Fungal
- Glycosylation
- Models, Biological
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Peptide Fragments
- Precipitin Tests
- Protein Precursors
- Protein Processing, Post-Translational