Article
The activity of a model heterologous protein in pep4-3 mutants of Saccharomyces cerevisiae.
Applied microbiology and biotechnology - 1 Mar 1992
Wingfield J M, Dickinson J R
Abstract excerpt
The bacterial lacZ gene was introduced into two sibling strains of Saccharomyces cerevisiae, one a wild-type strain with normal proteinase activity and the other a pep4-3 mutant strain. The pep4-3 mutation resulted in 90% reduced activity of the four major vacuolar proteinases. By comparing the a...
Topics
- Endopeptidases
- Lac Operon
- Mutation
- Plasmids
- Recombinant Proteins
- Saccharomyces cerevisiae
- Vacuoles
- beta-Galactosidase