Article
Sensitivity to ionizing radiation in Saos-2 cells transfected with mutant p53 genes depends on the mutation position.
Journal of radiation research - 1 Jun 1998
Okaichi K, Wang L H, Ihara M, Okumura Y
Abstract excerpt
We have constructed an in vitro system to examine how p53 mutants affect radiosensitivity. Mutations of p53 were made using in vitro mutagenesis, and mutant cDNAs were introduced into the human osteosarcoma cell line, Saos-2, which is devoid of endogenous p53. For wild type p53, both the expressi...
Topics
- Genes, p53
- Humans
- Mutation
- Osteosarcoma
- Radiation Tolerance
- Transfection
- Tumor Cells, Cultured