Article
Transfected mutant p53 gene increases X-ray-induced cell killing and mutation in human fibroblasts immortalized with 4-nitroquinoline 1-oxide but does not induce neoplastic transformation of the cells.
International journal of cancer - 29 Mar 1995
Kawashima K, Mihara K, Usuki H, Shimizu N, Namba M
Abstract excerpt
We introduced the mutant p53 gene (codon 273Arg-His) into human fibroblasts (SUSM-I cells) previously immortalized with 4-nitroquinoline I-oxide (4NQO) and obtained 2 clonal cell lines (SUSM-i/p53-1 and SUSM-1/p53-6) expressing the mutant p53. Since the genetic background of SUSM-1/p53 is the same as that of SUSM-1 except for the presence of the mutant p53, we expected to obtain more information on the mechanisms...
Topics
- 4-Nitroquinoline-1-oxide
- Animals
- Base Sequence
- Cell Death
- Cell Division
- Cell Line, Transformed
- Cell Transformation, Neoplastic
- Fibroblasts
- Genes, p53
- Humans