Article
Bisulfite sequencing in preimplantation embryos: DNA methylation profile of the upstream region of the mouse imprinted H19 gene.
Genomics - 15 Jul 1998
Warnecke P M, Mann J R, Frommer M, Clark S J
Abstract excerpt
In this study we describe a modification of the bisulfite genomic sequencing protocol that enables detection of methylation from as few as five diploid cells from preimplantation mouse embryos. We have used bisulfite genomic sequencing to study the methylation profile of the putative imprinting e...
Topics
- Alleles
- Animals
- Base Sequence
- Blastocyst
- Cytosine
- DNA Methylation
- Genomic Imprinting
- Mice
- Mice, Inbred Strains
- Molecular Sequence Data
- Muscle Proteins
- Polymerase Chain Reaction
- RNA, Long Noncoding
- RNA, Untranslated
- Sequence Analysis, DNA
- Sulfites