Article
The proofreading pathway of bacteriophage T4 DNA polymerase.
The Journal of biological chemistry - 4 Sept 1998
Reha-Krantz L J, Marquez L A, Elisseeva E, Baker R P, Bloom L B, Dunford H B, Goodman M F
Abstract excerpt
The base analog, 2-aminopurine (2AP), was used as a fluorescent reporter of the biochemical steps in the proofreading pathway catalyzed by bacteriophage T4 DNA polymerase. "Mutator" DNA polymerases that are defective in different steps in the exonucleolytic proofreading pathway were studied so th...
Topics
- 2-Aminopurine
- Bacteriophage T4
- Binding Sites
- DNA Primers
- DNA Replication
- DNA, Single-Stranded
- DNA-Directed DNA Polymerase
- Exonucleases
- Flow Injection Analysis
- Fluorescent Dyes
- Heparin
- Magnesium
- Models, Genetic
- Mutation
- Oligodeoxyribonucleotides
- Viral Proteins