Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities.

Abstract excerpt

Many DNA polymerases are multifunctional with the ability to replicate DNA as well as to proofread misincorporated nucleotides. Since polymerase and 3'--> 5' exonuclease activities appear to reside in spatially distinct active centers, there must be some mechanism for coordinating replication with proofreading and for transferring DNA between the two active centers. We have designed a genetic selection scheme to...

Topics

• Amino Acids
• Base Sequence
• Binding Sites
• DNA Primers
• DNA, Bacterial
• DNA, Circular
• DNA, Single-Stranded
• DNA-Directed DNA Polymerase
• Deoxyribonucleotides
• Exodeoxyribonuclease V