Article
Use of genetic analyses to probe structure, function, and dynamics of bacteriophage T4 DNA polymerase.
Methods in enzymology - 1 Jan 1995
Reha-Krantz L J
Abstract excerpt
Functionally distinct mutant DNA polymerases have been isolated by the genetic selection strategies described here. These methods can be supplemented by the use of targeted mutagenesis procedures to enhance mutagenesis of DNA polymerase genes and to direct mutagenesis to specific sites in cloned...
Topics
- Amino Acid Sequence
- Bacteriophage T4
- Base Sequence
- DNA Replication
- DNA, Viral
- DNA-Directed DNA Polymerase
- Diphosphates
- Enzyme Inhibitors
- Genes, Lethal
- Genes, Viral
- Molecular Sequence Data
- Mutagenesis
- Mutagenesis, Insertional
- Mutagenesis, Site-Directed
- Phenotype
- Sequence Deletion
- Temperature
- Viral Proteins