An efficient system for site-directed mutagenesis to make various mutants of the env gene of human immunodeficiency virus type 1.

doi:10.1016/s0014-5793(98)00687-5

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Article

An efficient system for site-directed mutagenesis to make various mutants of the env gene of human immunodeficiency virus type 1.

FEBS letters - 3 Jul 1998

Shimizu N, Hoshino H

Abstract excerpt

We developed an efficient system of site-directed mutagenesis for the envelope (env) gene of human immunodeficiency virus type 1 (HIV-1). To make a template plasmid for mutagenesis, pS+B/MluI, two independent selection markers, i.e. a unique restriction site, MluI, and an in-frame termination cod...

Topics

Amino Acid Sequence
Base Sequence
Genes, env
HIV Envelope Protein gp120
HIV-1
Humans
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Peptide Fragments
Plasmids
Polymerase Chain Reaction

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