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Article

An efficient system for site-directed mutagenesis to make various mutants of the env gene of human immunodeficiency virus type 1.

FEBS letters - 3 Jul 1998

Shimizu N, Hoshino H

Abstract excerpt

We developed an efficient system of site-directed mutagenesis for the envelope (env) gene of human immunodeficiency virus type 1 (HIV-1). To make a template plasmid for mutagenesis, pS+B/MluI, two independent selection markers, i.e. a unique restriction site, MluI, and an in-frame termination codon, were introduced into the region encoding the V3 domain of the env gene of an HIV-1 strain, NL4-3, which had been...

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Identifiers

PMID: 9688566
DOI: 10.1016/s0014-5793(98)00687-5

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Source receipt: pubmed, retrieved 7 Sept 2026.

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