Introduction of site-specific mutations into the genome of influenza virus.

doi:10.1073/pnas.87.10.3802

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Article

Introduction of site-specific mutations into the genome of influenza virus.

Proceedings of the National Academy of Sciences of the United States of America - 1 May 1990

Enami M, Luytjes W, Krystal M, Palese P

Abstract excerpt

We succeeded in rescuing infectious influenza virus by transfecting cells with RNAs derived from specific recombinant DNAs. RNA corresponding to the neuraminidase (NA) gene of influenza A/WSN/33 (WSN) virus was transcribed in vitro from plasmid DNA and, following the addition of purified influenz...

Topics

Animals
Base Sequence
Cell Line
DNA-Directed RNA Polymerases
Genes, Viral
Influenza A virus
Molecular Sequence Data
Mutation
Oligonucleotide Probes
Plasmids
RNA, Viral
Ribonucleoproteins
Transfection

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