Article
[A method for introducing mutations into large vectors].
Zhongguo fei ai za zhi = Chinese journal of lung cancer - 20 Jul 2014
Meng Fanrong, Chen Chen, Wan Haisu, Zhou Qinghua
Abstract excerpt
BACKGROUND AND OBJECTIVE: In vitro site-directed mutagenesis is a routine technique in molecular biology labs. However, although there are numbers of related methods available, most of these methods are not suitable for introducing mutations into large vectors. METHODS: In this report, we describe a method which is highly effective for this purpose. Our method is based on the other site-directed method we...
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