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Article

Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1

1 Feb 1992

Abstract excerpt

We constructed a series of BspMI cassettes that simplify the introduction of specific point mutations in the polymerase domain of human immunodeficiency virus type 1 reverse transcriptase. A series of point mutants were constructed by using these cassette vectors. The RNA-dependent DNA polymerase and RNase H activities of 20 point mutations in the conserved portion of the polymerase domain were assayed. All the...

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Identifiers

PMID: 1370546
DOI: 10.1128/jvi.66.2.1031-1039.1992

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