Article
Construction and characterization of expression systems for the env gene of ts1, a mutant of Moloney murine leukemia virus-TB.
Virus research - 1 Mar 1991
Yu Y, Kamps C A, Yuen P H, Wong P K
Abstract excerpt
A temperature-sensitive mutant of the Moloney murine leukemia virus-TB, ts1, causes hindlimb paralysis and immunodeficiency in mice. At the restrictive temperature, the envelope precursor polyprotein, gPr80env, is inefficiently processed intracellularly, and this is associated with the neurovirulence of ts1. To test the hypothesis that expression of the envelope proteins of ts1 alone without infectious virus...
Topics
- Animals
- Cloning, Molecular
- Gene Expression
- Gene Products, env
- Genes, env
- Mice
- Moloney murine leukemia virus
- Mutation
- Plasmids
- Repetitive Sequences, Nucleic Acid
- Transfection