Article
High affinity Ca2+ binding sites of calmodulin are critical for the regulation of myosin Ibeta motor function.
The Journal of biological chemistry - 7 Aug 1998
Zhu T, Beckingham K, Ikebe M
Abstract excerpt
We coexpressed myosin Ibeta heavy chain with three different calmodulin mutants in which the two Ca2+-binding sites of the two N-terminal domain (E12Q), C-terminal domain (E34Q), or all four sites (E1234Q) are mutated in order to define the importance of these Ca2+ binding sites to the regulation...
Topics
- Actins
- Adenosine Triphosphatases
- Animals
- Binding Sites
- Calcium
- Calcium-Binding Proteins
- Calmodulin
- Calmodulin-Binding Proteins
- Cattle
- Models, Molecular
- Mutation
- Myosin Heavy Chains
- Protein Binding
- Protein Conformation
- Recombinant Proteins