Article
Calcium-dependent regulation of the motor activity of recombinant full-length Physarum myosin.
Journal of biochemistry - 1 Aug 2012
Zhang Ying, Kawamichi Hozumi, Tanaka Hideyuki, Yoshiyama Shinji, Kohama Kazuhiro, Nakamura Akio
Abstract excerpt
We successfully synthesized full-length and the mutant Physarum myosin and heavy meromyosin (HMM) constructs associated with Physarum regulatory light chain and essential light chain (PhELC) using Physarum myosin heavy chain in Sf-9 cells, and examined their Ca(2+)-mediated regulation. Ca(2+) inh...
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