Identification of deletions and insertions in the p53 gene using multiplex PCR and high-resolution fragment analysis: application to breast and ovarian tumors.

Abstract excerpt

We have developed a simple and highly efficient method to detect deletions and insertions in the p53 gene. All 11 exons of the p53 gene were amplified along with a control sequence in four multiplex PCR reactions in the presence of fluorescein-labeled primers. The PCR products were resolved on an...

Topics

• Breast Neoplasms
• Cloning, Molecular
• DNA Transposable Elements
• DNA, Neoplasm
• Exons
• Female
• Genes, p53
• Humans
• Introns
• Mutation
• Ovarian Neoplasms
• Polymerase Chain Reaction
• Sequence Analysis, DNA
• Sequence Deletion