Article
Generation of transgenic mice.
Current protocols in cell biology - 1 Mar 2009
Cho Andrew, Haruyama Naoto, Kulkarni Ashok B
Abstract excerpt
This unit describes detailed step-by-step protocols, reagents, and equipment required for successful generation of transgenic mice using pronuclear injection. The experimental methods and practical tips given here will help guide beginners in understanding what is required and what to avoid in these standard protocols for efficiently generating transgenic mice.
Topics
- Animals
- Embryo Implantation
- Genetic Engineering
- Genotype
- Mice
- Mice, Transgenic
- Nuclear Transfer Techniques
- Transgenes