Article
Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions.
Nucleic acids research - 22 Mar 2005
Aoyama Minako, Agari Kazuko, Sun-Wada Ge-Hong, Futai Masamitsu, Wada Yoh
Abstract excerpt
In a gene targeting experiment, the generation of a targeting construct often requires complex DNA manipulations. We developed a set of cassettes and plasmids useful for creating targeting vectors to modify the mammalian genome. A positive selection marker cassette (PGK/EM7p-npt), which included dual prokaryotic and eukaryotic promoters to permit consecutive selection for recombination in Escherichia coli and...
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