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Article

Generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes.

Proceedings of the National Academy of Sciences of the United States of America - 1 Feb 1990

Pavan W J, Hieter P, Reeves R H

Abstract excerpt

Mammalian DNA segments cloned as yeast artificial chromosomes (YACs) can be manipulated by DNA-mediated transformation when placed in an appropriate yeast genetic background. A "fragmenting vector" has been developed that can introduce a yeast telomere and selectable marker into human-derived YACs at specific sites by means of homologous recombination, deleting all sequences distal to the recombination site. A...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 2406718
DOI: 10.1073/pnas.87.4.1300
PMCID: PMC53462

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Source receipt: pubmed, retrieved 7 Sept 2026.

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