Generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes.

doi:10.1073/pnas.87.4.1300

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Article

Generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes.

Proceedings of the National Academy of Sciences of the United States of America - 1 Feb 1990

Pavan W J, Hieter P, Reeves R H

Abstract excerpt

Mammalian DNA segments cloned as yeast artificial chromosomes (YACs) can be manipulated by DNA-mediated transformation when placed in an appropriate yeast genetic background. A "fragmenting vector" has been developed that can introduce a yeast telomere and selectable marker into human-derived YAC...

Topics

Chromosome Deletion
Chromosomes, Fungal
Cloning, Molecular
Escherichia coli
Genes, Bacterial
Humans
Phenotype
Plasmids
Restriction Mapping
Saccharomyces cerevisiae
Transformation, Genetic

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