Article
Drosophila acetylcholinesterase: effect of post-translational [correction of post-traductional] modifications on the production in the baculovirus system and substrate metabolization.
Archives of insect biochemistry and physiology - 1 Jan 1998
Estrada-Mondaca S, Lougarre A, Fournier D
Abstract excerpt
Acetylcholinesterase cDNAs from Drosophila melanogaster modified on its primary sequence were cloned into baculovirus and were expressed in Sf9 cells with the aim to identify a mutant form that produces the enzyme at a high level. Directed mutagenesis was used in order to independently knockout d...
Topics
- Acetylcholine
- Acetylcholinesterase
- Animals
- Baculoviridae
- Cloning, Molecular
- Cysteine
- DNA, Complementary
- Drosophila melanogaster
- Gene Expression Regulation, Enzymologic
- Kinetics
- Mutagenesis, Site-Directed
- Mutation
- Protein Processing, Post-Translational