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Article

Expression of recombinant acetylcholinesterase in a baculovirus system: kinetic properties of glutamate 199 mutants.

Biochemistry - 13 Oct 1992

Radić Z, Gibney G, Kawamoto S, MacPhee-Quigley K, Bongiorno C, Taylor P

Abstract excerpt

The glycophospholipid-linked, amphiphilic form of acetylcholinesterase (AChE) from Torpedo californica and the hydrophilic form from mouse were overexpressed in Sf9 insect cells using the baculovirus expression system. Recombinant baculovirus, constructed by inserting AChE cDNA's into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the strong polyhedron promoter, yielded recombinant...

Topics

Acetylcholinesterase
Animals
Baculoviridae
Cells, Cultured
Cholinesterase Inhibitors
Glutamates
Glutamic Acid
Glycosylation
Kinetics
Mice
Moths

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Recombinant Proteins

Substrate Specificity

Torpedo

X-Ray Diffraction

Source

Identifiers

PMID: 1356436
DOI: 10.1021/bi00155a032

Genes

SDS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1356436-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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