Article
A mutation in a single gene of Schizosaccharomyces pombe affects the expression of several snRNAs and causes defects in RNA processing.
The EMBO journal - 1 Feb 1990
Potashkin J, Frendewey D
Abstract excerpt
A bank of temperature sensitive (ts-) mutants of Schizosaccharomyces pombe was screened for snRNA expression mutants using an oligodeoxynucleotide that recognizes U2 RNA. One mutant with a novel phenotype was identified that has reduced steady-state levels of the spliceosomal snRNAs U1, U2, U4, U...
Topics
- Base Sequence
- Blotting, Northern
- Gene Expression
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- RNA Precursors
- RNA Splicing
- RNA, Catalytic
- RNA, Fungal
- RNA, Ribosomal
- RNA, Small Nuclear
- Restriction Mapping
- Ribonucleases
- Saccharomycetales
- Schizosaccharomyces
- Temperature