Article
Detection and quantification of insertion/deletion variations by allele-specific real-time PCR: application for genotyping and chimerism analysis.
Biological chemistry - 1 Sept 2002
Wilhelm Jochen, Reuter Hendrik, Tews Björn, Pingoud Alfred, Hahn Meinhard
Abstract excerpt
The DNA-based quantitative analysis of genetic chimerism is becoming increasingly more important for molecular biology in general and molecular medicine in particular. Useful genomic targets for these analyses are polymorphic sequences, but here the problem of a reliable quantification with high dynamic range is not yet satisfactorily solved. To this end we have combined the allele-specific amplification with a...
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