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Allelic discrimination of factor V Leiden using a 5' nuclease assay.

Thrombosis and haemostasis - 1 Oct 1999

Happich D, Schwaab R, Hanfland P, Hoernschemeyer D

Abstract excerpt

The G1691A (Leiden) mutation of the factor V gene is the most prevalent identified cause of venous thrombosis. Therefore, we developed a new genetic test using the TaqMan system. With this assay which combines PCR amplification and detection reaction in one closed tube, a cohort of 234 patients with a history of thrombosis was screened. In parallel, amplification products of the same patients were screened with a...

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PMID: 10544916

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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