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Article

A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates.

Cell - 2 Jul 1993

Burgess S M, Guthrie C

Abstract excerpt

PRP16 encodes an RNA-dependent ATPase required for the second step of mRNA splicing in S. cerevisiae. We have isolated seven alleles of PRP16 that, like the original allele prp16-1, allow splicing of introns with a mutant branch site (UACUAAC to UACUACC), by forming lariat intermediates at the mutant C nucleotide. Every suppressor mutation maps to the region of PRP16 common to RNA-dependent ATPases. We purified...

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PMID: 8324826
DOI: 10.1016/0092-8674(93)90363-u

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates.PMID 8324826Read ↗

A dominant negative mutation in a spliceosomal ATPase affects ATP hydrolysis but not binding to the spliceosome.Semantic proximity Read ↗

Characterization of Cwc2, U6 snRNA, and Prp8 interactions destabilized by Prp16 ATPase at the transition between the first and second steps of splicing.Semantic proximity Read ↗

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Genome-wide analysis of pre-mRNA splicing: intron features govern the requirement for the second-step factor, Prp17 in Saccharomyces cerevisiae and Schizosaccharomyces pombe.Semantic proximity Read ↗

Suppression of multiple substrate mutations by spliceosomal prp8 alleles suggests functional correlations with ribosomal ambiguity mutants.Semantic proximity Read ↗

Splicing proofreading at 5' splice sites by ATPase Prp28p.Semantic proximity Read ↗

The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro.Semantic proximity Read ↗

Opposing classes of prp8 alleles modulate the transition between the catalytic steps of pre-mRNA splicing.Semantic proximity Read ↗

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Genetic interactions between the yeast RNA helicase homolog Prp16 and spliceosomal snRNAs identify candidate ligands for the Prp16 RNA-dependent ATPase.Semantic proximity Read ↗

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