Article
A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates.
Cell - 2 Jul 1993
Burgess S M, Guthrie C
Abstract excerpt
PRP16 encodes an RNA-dependent ATPase required for the second step of mRNA splicing in S. cerevisiae. We have isolated seven alleles of PRP16 that, like the original allele prp16-1, allow splicing of introns with a mutant branch site (UACUAAC to UACUACC), by forming lariat intermediates at the mu...
Topics
- Adenosine Triphosphatases
- Alleles
- Amino Acid Sequence
- DNA Mutational Analysis
- Fungal Proteins
- Genes, Fungal
- Genes, Suppressor
- Molecular Sequence Data
- RNA Helicases
- RNA Splicing
- RNA Splicing Factors
- RNA, Messenger
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins